M-MLV Reverse Transcriptase from Moloney Murine Leukemia Virus which is kind of DNA polymerase can be used for synthesis of single stranded RNA, DNA or complementary DNA strand from RNA:DNA hybrid such as AMV RT. Especially, it is normally used for synthesis of cDNA from RNA and the endonulcease activity as well as RNase H activity is lower comparing to AMV RT.

1) Unit definition
One unit incorporates 1nmol dTTP into acid-precipitable material in 10min at 37C.

2) QC data
Nuclease activity is not detected.

3) Storage buffer
20mM Tris-HCl (pH 7.5)
0.1mM DTT
0.01% NP-40
0.1mM EDTA
0.1M NaCl
50% glycerol

4) General use
Use 1§¡ of M-MLV RT in a 20§¡ of reaction.

- cDNA synthesis, Sequencing single and double-stranded DNA, Sequencing single and double-stranded RNA, Random priming reaction