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Maxime™ RT PreMix Kit is the product what is mixed every component for
cDNA synthesis in each tube for 1 rxn PCR. Maxime™ RT PreMix Kit has two kinds of product,
that is, oligo (dT)15 primer and random primer- for choose the product for each using type.
The product that finished a cDNA synthesis reaction can do PCR directly by using
Maxime PCR PreMix Kit.
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Shot Protocol |
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Add only RNA template for RT.
Mixed with Oligo(dT)15 primer or Random primer in PCR tube.
Eliminate complicated procedure such as incubation step.
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High Purity Enzyme |
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DNA Polymerase, using the anion exchange chromatography,
so we can attain 95~99% of pure polymerase.
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Convenience |
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Maxime RT-PreMix Kit is mixed every component. RTase, dNTP mixture,
reaction buffer, Oligo dt or Random primer and so on in one tube for 1 rxn PCR.
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Reproducibility |
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We quoted ISO 9001 and 14001, as perform special QC, we guarantee regular quality.
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Guarantee Date |
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Store at -20 °C for one year. |
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| Comparison with different kit
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Total RNA was purified from mouse cells using easy-BLUE™ Total RNA Extraction Kit (Cat. No. 17061). And then, the first strand of cDNA was synthesized using and Maxime RT PreMix Kit (Oligo dT Primer) and company’s A RT PreMix Kit.
After diluting the cDNA mixture as indicates, the PCR reaction was performed using Maxime™ PCR PreMix Kit
Comparison of Maxime PCR PreMix (Oligo dT Primer) and Company A's RT PreMix system by amplifying 570bp DNA fragment (GAPDH).
Lane M, 100bp Ladder DNA Marker Lane 1, (1/2)3 diluted cDNA
Lane 2, (1/2)4 diluted cDNA Lane 3, (1/2)5 diluted cDNA
Lane 4, (1/2)6 diluted cDNA Lane 5, (1/2)7 diluted cDNA |
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