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  HOME > Plasmid & Probe DNA > PCR & Agarose > MEGAquick-spin¢â PCR & Agarose Gel DNA Extraction Kit
 
¢Æ MEGAquick-spin¢â PCR & Agarose Gel DNA Extraction Kit
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Ordering Information  
Cat. No Size Price Order
17282 250 col. $0 (USD)
17281 50 col. $0 (USD)
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Description
The MEGAquick-spin¢â PCR & Agarose Gel DNA Extraction Kit is designed to recover or concentrate DNA fragments (87 bp~20 kb) from agarose gels, PCR or other enzymatic reactions. Up to 95% recovery is achieved depending upon the fragment size. PCR products are commonly purified to remove excess nucleotides and primers.
Characteristics
1) Allows purification directly from PCR reactions or agarose gel slices
2) Purify DNA fragments or PCR products in as little as 20 minutes.
3) Up to 95% recovery of ready-to-use DNA.
4) Purify DNA fragments (87 bp~20 kb).
5) Purified DNA fragments are ready to use for PCR, sequencing, restriction digestion.

Simple protocol
EXPERIMENTAL INFORMATION
1. Complete primer removal after PCR product
Figure. The bar-graph shows the recovery of DNA fragment. The values of yield was estimated with TINA2.0 software.

Before : Before purification
Control : PCRquick-spinTM PCR Product Purification Kit(iNtRON)
iNtRON : MEGAquick-spinTM PCR & Agarose Gel DNA Extraction System
Supplier Q and P : Q and P company products

Lane M. 100 bp Ladder molecular weight DNA marker
Lane 1. Multiplex PCR purification
Lane 2. 570 bp DNA fragment purification
Lane 3. 1.0 kb DNA fragment purification

2. Adequate DNA recovery
Figure. The bar-graph shows the recovery of DNA fragment. The values of yield was estimated with TINA2.0 software.

Before : Before purification
Control : PCRquick-spinTM PCR Product Purification Kit(iNtRON)
iNtRON : MEGAquick-spinTM PCR & Agarose Gel DNA Extraction System
Supplier Q and P : Q and P company products

Lane M. 1 kb Ladder molecular weight DNA marker
Lane 1. 570 bp DNA fragment extraction
Lane 2. 1.3 kb DNA fragment extraction
Lane 3. 4.5 kb DNA fragment extraction


3. Maximum Yield
The first fraction(Lane 4.) showed app. 80% of recovery, but the recovery was increased up to 95% by repeated elution.

Figure. The additional elution for recovery enhancing.
After 1st elution, another elution was repeated by adding elution buffer, newly.

Lane M. 1 kb Ladder Molecular Weight DNA marker
Lane 1. 100% of DNA
Lane 2. 20% of DNA
Lane 3. 10% of DNA
Lane 4. 1st eluate
Lane 5. 2nd eluate
Lane 6. 3rd eluate
Lane 7. 4th eluate


 
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