iNtRON Biotechnology

Plasmid & Probe DNA

Genomic DNA Prep

RNA Prep

Protein

PCR

RT/RT-PCR

DNA & Protein Markers

Chemical & Other

Cloning & Enzyme

Molecular Diagnostics

HOME > PCR > Aliquoted & Dried PreMix > Maxime™ PCR PreMix Kit ( i-MAX II)

Untitled Document

Maxime™ PCR PreMix Kit (i-MAX II) ‘s characteristics are there. Increased fidelity of PCR amplification due to the i-MAX™ II DNA Polymerase enzyme blend combines the proofreading activity of Pfu DNA Polymerase with the high processivity of Taq DNA Polymerase. The second is that increased yield of PCR amplification.
Finally, improved performance of long PCR because the reaction buffer and the enzyme blend are optimized for generation of certain length products. So, it can amplify even longer fragments up to 17.5kb from human genomic DNA, and up to 30kb from a DNA template.
Maxime™ PCR PreMix (i-MAX II) is the product what is mixed every component: i-MAX™ II DNA Polymerase, dNTP mixture, reaction buffer, and so on- in one tube for 1 rxn PCR. This is the product that can get the best result with the most convenience system. The first reason is that it has every components for PCR, so we can do PCR just add a template DNA, primer set, and D.W.. The second reason is that it has Gel loading buffer to do electrophoresis, so we can do gel loading without any treatment. In addition, each batches are checked by a thorough Q.C., so its reappearance is high.
It is suitable for various sample's experience by fast and simple using method.

	Increased fidelity of PCR amplification
	Increased yield of PCR amplification
	: Because the accuracy of the enzyme blend reduces the number of truncated amplification products formed.
	Improved performance of long PCR
	Ready to use: only template and primers are needed
	Stable for over 1 year at -20℃
	Time-saving and cost-effective

Experimental Information for Long PCR, Multi-Plex, Normal condition

Amplification of various templates 87 bp to 20 Kb.

[ Amplification of various template 87bp to 20Kb with Maxime™ PCR PreMix Kit (i-Max II) ]
Lane M1, 100 bp Ladder DNA Marker; Lane M2, 1 kb Ladder DNA Marker;
L ane 1, 87 bp; Lane 2, 200 bp; Lane 3, 570 bp, Lane 4, 1 kb; Lane 5, 1.3 kb;
Lane 6, 1.8 kb; Lane 7, 2 kb; Lane 8, 2.7 kb; Lane 9, 4.5 kb; Lane 10, 9 kb;
Lane 11, 17.5 kb; Lane 12, 20 kb

Amplification of various template 97bp to 20Kb with Maxime™ PCR PreMix Kit (i-Max II)

Comparision with other company

[ Using only human gDNA template ]
Lane M1, 100bp Ladder DNA Marker; Lane M2, λ/Hind III Marker;
L ane 1, 1.8Kb; Lane 2, 2Kb; Lane 3, 2.7Kb, Lane 4, 17.5Kb