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Many plant viruses have RNA genomes, either single stranded (ss) RNA or double stranded (ds) RNA. In addition, many viruses but virus-like agent found in plant pathogenic fungi have dsRNA genomes and there is increasing interest in analyzing such fungi for dsRNAs. RNA viral genomes often segmented and the number and size of these segments is represented in an infected plant either as a population of ss RNAs destined to act as a template for replication, as mRNAs destined to act in protein synthesis, as viral genomes to be encapsidated, or as equivalent dsRNAs, destined to be or to have been involved as intermediaries in replication.
The dsRNAs representing known genomic and subgenomic ssRNAs and additional virus specific dsRNAs of even more uncertain origin are not present in equal amount, and the most abundant ones seem to be those that correspond to the genomic ssRNAs. The presence, number, size, and abundance of major and minor dsRNAs for a specific virus form a collection of measurable properties which are of potential value for diagnosis.
Plant pathogenic viral dsRNA isolation kit(for plant issue) is realized that the specific isolation of plant pathogen and the simplification of those experimental procedure. Thus, it is promising to improve your plant disease diagnosis.
Lysis Buffer : Store at 4oC, after receiving.
Other components : Store at Room temperature
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