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i-StarMAX™ II DNA Polymerase is an optimal enzyme mixture of i-StarTaq™ DNA Polymerase and a proofreading DNA polymerases. i-StarTaq™ DNA Polymerase is a thermostable, modified form of recombinant Taq DNA Polymerase suitable for hot start PCR experiments. As a result of addition of i-StarTaq™ DNA Polymerase, i-StarMAX™ II DNA Polymerase cannot be useful tool only in amplification of short and long fragments but also problematic template/primer systems.
High yields of PCR product can be achieved using extension times as short as from 30 seconds to 1 minute per kb per cycle with the i-StarMAX™ II DNA Polymerase . The i-StarMAX™ II DNA Polymerase is recommended for relatively rapid, high-fidelity amplification of PCR targets up to 15kb when proofreading DNA polymerase alone requires too long an extension time or yields are insufficient. |
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- High fidelity, specificity, and yields
- Versatile for various DNA template including cloned fragment, phage DNA, mammalian genomic DNA and etc.
- Ideal for difficult templates, such as GC-rich or looped sequences |
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- Amplification of genomic DNA and cDNA targets up to 15kb long with high specificity, sensitivity, and yield
- PCR with difficult template/primer system
- Multi-plex PCR
- Cloning with TA and blunt ends |
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i-StarMAXTM II DNA Polymerase
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50 units (5U/ml)
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10X PCR buffer (20mM Mg2+)
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1.5 ml
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10X Mg2+free buffer
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1.5 ml
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10mM dNTPs (2.5mM each)
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800 ml
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25mM Mg2+
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1.5ml
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Stored at -20℃ (Stable for 1 year) |
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 Amplification of various Hot-Start PCR condition |
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그림 1. Amplification of various Hot-Start PCR condition
[Lane M] 100 bp Ladder DNA Marker, [Lane a] 100 ng genomic DNA
[Lane b] 5-1 diluted genomic DNA, [Lane c] 5-2 diluted genomic DNA
[Lane d] 5-3 diluted genomic DNA, Lane e] 5-4 diluted genomic DNA |
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그림 2. Amplification of various Hot-Start PCR condition
[Lane M] 100 bp Ladder DNA Marke
[Lane a] : 161 bp, [Lane b] : 165 bp, [Lane c] : 166 bp
[Lane d] : 181 bp, [Lane e] : 218 bp, [Lane f] : 266 bp |
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