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HOME > PCR > DNA polymerase > i-Taq™ plus DNA Polymerase

The polymerase chain reaction(PCR) is an enzyme-mediated gene amplification technique. PCR is now a common tool used in medical and biological research labs for a variety of tasks, such as sequencing of genes, cloning of genes, the diagnosis of hereditary diseases, the identification of genetic fingerprints (used in forensics and paternity testing), the detection and diagnosis of infectious diseases, and the creation of transgenic organisms. In the beginning, the enzyme used in PCR was E.coli DNA polymerase, and this enzyme had to be added at every step of the process due to its thermal instability. Since Taq DNA polymerase was developed from Thermus aquaticus bacteria which thrives in hot spa, the contemporary automatic PCR has been available. Taq DNA polymerase optimally synthesizes DNA at 72 ℃, therefore it could stably amplify a specified oligo sequence without adding enzyme at every step due to its thermal stability even at 94℃. iNtRON i-Taq™ Plus DNA Polymerase is suitable for standard PCR and special PCR applications.
Specially designed i-Taq™ Plus reaction buffer provides robust performance for reproducible results in a various type of PCR applications as well as high efficiency and high specificity in PCR.

- High efficiency and high specificity of amplification due to specially designed PCR buffer
- Applicable to general PCR, RT-PCR, differential display, multiplex PCR, and PCR-based DNA fingerprinting (VNTR, STR, and RAPD)

- Genomic DNA PCR
- RT-PCR
- Direct sequencing related PCR
- T/A vector cloning
- LOH or MSI analysis related PCR

i-Taq™ Plus DNA Polymerase (5U/ml)

250 units (500 units)

10x i-Taq™ Plus PCR buffer

1ml

10x i-Taq™ Plus MgC₂ free PCR buffer

1ml

10mM dNTPs (2.5mM each)

500ul (1ml)

25mM MgCl₂

1ml

10X i-Taq™ Plus PCR BUFFER

350mM Tris-HCL (pH 9.0)
250mM KCL
35mM MgCl₂
Enhancer solution

-20℃ 보관

Template DNA (optimal amounts of templates from different origins)

Starting template	Optimal range
Human genomic DNA cDNA Bacterial DNA PCR fragment (1 kbp DNA) Plasmid DNA	1 ng ~ 200 ng 10 ng ~ 100 ng 10 pg ~ 10 ng 10 fg ~ 1 ng 0.1 ng ~ 50 ng

Amplification Efficiency

Figure 1. Amplification efficiency of i-Taq™ Plus DNA Polymerase.
Each DNA fragment is amplified from K562 genomic DNA extracted by iNtRON i- genomic CTB DNA Extraction Mini Kit (Cat. No. 17341).

Figure 2. Comparison of amplification efficiency with other company’s products.
The templates used in PCR are human genomic DNA.
Lane M, 1 kb DNA ladder;
L ane 1, 200 bp p53 gene fragment amplified from 12.5 ng gDNA;
L ane 2, 360 bp TNF-α gene fragment amplified from 6.25 ng gDNA;
L ane 3, 1.1 kbp IL-10 gene fragment amplified from 12.5 ng gDNA;
Lane 4, 1.8 kbp α-globin gene fragmente from 25 ng gDNA